极地研究 ›› 2017, Vol. 29 ›› Issue (1): 74-81.DOI: 10.13679/j.jdyj.2017.1.074

• 研究论文 • 上一篇    下一篇

持续光照下光强对北极海域芬迪亚历山大藻生长及产毒的影响

吕意华, 熊小飞, 吴加欣, 杨振雄, 杨熙, 董燕红   

  1. 国家海洋局南海分局南海环境监测中心, 广东 广州 510300
  • 收稿日期:2015-11-05 修回日期:2016-03-21 出版日期:2017-03-30 发布日期:2017-03-30
  • 通讯作者: 吕意华
  • 基金资助:

    海洋赤潮灾害立体监测技术与应用国家海洋局重点实验室开放研究基金(MATHAB201604); 海洋公益性行业科研专项(201305010-2)

Influence of irradiation on the paralytic shellfish toxin content and profiles of Alexandrium tamarense isolated #br# from the Arctic waters

Lv Yihua, Xiong Xiaofei, Wu Jiaxin, Yang Zhenxiong, Yang Xi, Dong Yanhong   

  • Received:2015-11-05 Revised:2016-03-21 Online:2017-03-30 Published:2017-03-30
  • Contact: Yihua Lyu

摘要:

以格陵兰岛西部、冰岛沿海和楚科奇海的芬迪亚历山大藻为受试对象, 并以缅因湾的芬迪亚历山大藻藻株做为参照, 从沉积物或海水中的休眠孢囊或营养细胞中分离到12株目标藻种, 并在实验室建立纯培养系。然后对这些地域的藻种在不同光照下生长特性的比较、对毒素含量进行研究, 发现芬迪亚历山大藻在持续光照条件下最适的生长光强范围是38—91 µmol·m–2·s–1。另外, 在所有分离到的12株藻细胞中均未检测到脱氨甲酰基膝沟藻毒素2(dcGTX2), 大部分藻细胞都在光强较强时, 即生长速率低时表现出更高的细胞毒素含量和毒性。

关键词: 芬迪亚历山大藻, 北极, 缅因湾, 光照, 麻痹性贝毒

Abstract:

A comparative analysis of the physiology and toxin composition was performed on a suite of clonal cultures of the potentially toxic dinoflagellate Alexandrium tamarense. These were established from resting cysts or vegetative cells isolated from sediment or the water taken from the coasts of Greenland, Iceland, Chukchi Sea and Gulf of Maine. Maximum growths were observed between 38 µmol·m–2·s–1 and 91 µmol·m–2·s–1. No dcGTX2 was detected in all 12 isolates, toxin content significant variation was observed. In general, toxin contents and toxicity were generally highest at higher irradiation. This may indicate that the genetically determined toxin profiles in A. tamarense species are more complex than previously appreciated.

Key words: Alexandrium tamarense, the Arctic, Gulf of Main, Irradiation, PSP toxins